Which is the proper procedure for preparation of Platelets from Whole Blood?

The proper procedure for the preparation of platelets from whole blood primarily involves using a centrifugation method known as a light spin followed by a hard spin. The initial light spin is designed to separate the blood components based on their density, allowing the plasma, which contains platelets, to be separated from the heavier red blood cells and white blood cells. Following this, a hard spin is performed, which further concentrates the platelets.

The sequence of these spins is critical as it ensures the effective separation of platelets while minimizing their activation and maintaining their viability for transfusion. By first utilizing a light spin, the plasma and cellular components can be adequately separated without damaging the platelets. The subsequent hard spin then efficiently collects the platelets from the plasma. This approach maximizes platelet yield and preserves their functional properties for clinical use.

Other methods, such as two hard spins or a hard spin followed by a light spin, do not align with the standard practices for platelet preparation, possibly leading to suboptimal recovery or quality of the platelets.